Primary Culture System for Germ Cells from Caenorhabditis elegans Tumorous Germline Mutants

The Caenorhabditis elegans germ line is an important model system for the study of germ stem cells. Wild-type C. elegans germ cells are syncytial and therefore cannot be isolated in in vitro cultures. In contrast, the germ cells from tumorous mutants can be fully cellularized and isolated intact fro...

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Bibliographic Details
Published in:Bio-Protocol
Main Authors: Alexandra Vagasi, Mohammad Rahman, Snehal Chaudhari, Edward Kipreos
Format: Article
Language:English
Published: Bio-protocol LLC 2017-08-01
Online Access:https://bio-protocol.org/en/bpdetail?id=2424&type=0