Improving the Efficiency of CRISPR/Cas9-Mediated Non-Homologous End Joining Gene Knockout Using Small Molecules in Porcine Cells

The CRISPR/Cas9 genome editing system has emerged as an effective platform to generate loss-of-function gene edits through non-homologous end joining (NHEJ) without a repair template. To verify whether small molecules can enhance the efficiency of CRISPR/ Cas9-mediated NHEJ gene editing in porcine c...

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Bibliographic Details

Published in:	Biomolecules
Main Authors:	Shihao Lv, Xiaokang Xu, Sijia Yang, Mingjie Feng, Zhongyu Yuan, Xueqing Liu, Chaoqian Jiang, Jun Song, Yanshuang Mu
Format:	Article
Language:	English
Published:	MDPI AG 2025-08-01
Subjects:	<i>Sus scrofa</i> CRISPR/Cas9 small molecule editing efficiency NHEJ
Online Access:	https://www.mdpi.com/2218-273X/15/8/1132