Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFv

Summary: Fluorescent labeling of proteins is a widespread approach for the microscopic examination of protein function, expression, and localization in the cell. Here, we present a protocol for the labeling of hemagglutinin (HA)-tagged protein of interest (POI) with the single-chain antibody (scFv)...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Ioannis Tsirkas, Tomer Zur, Daniel Dovrat, Zohar Paleiov, Lior Ravkaie, Amir Aharoni
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Subjects:	Cell Biology Single Cell Microscopy Molecular/Chemical Probes
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723002666