Cofactor engineering for improved production of 2,4-dihydroxybutyric acid via the synthetic homoserine pathway

(L)-2,4-dihydroxybutyrate (DHB) is a versatile compound that can serve as a precursor for the synthesis of the methionine analog 2-hydroxy-4-(methylthio)butyrate and new advanced polymers. We previously implemented in Escherichia coli an artificial biosynthetic pathway for the aerobic production of...

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Bibliographic Details

Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Nadine Ihle, Laura Grüßner, Ceren Alkim, T. A. Stefanie Nguyen, Thomas Walther, Cláudio J. R. Frazão
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-02-01
Subjects:	enzyme engineering strain engineering cofactor specificity synthetic metabolic pathway 2,4-dihydroxybutyric acid homoserine
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2025.1504785/full