Cloning and sequencing of the S gene of H52 strain from avian infectious bronchitis virus
Virion RNA from H52 strain of avian Infectious Bronchitis were extracted from the allantoic fluid of inoculated embryonated eggs and used as template for cDNA synthesis by reverse transcription with 3'-primer. Polymerase chain reaction was performed with two primers which span the S gene, and a...
| Published in: | 浙江大学学报. 农业与生命科学版 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2002-05-01
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| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2002.03.0303 |