Protocol for generation and engineering of thyroid cell lineages using CRISPR-Cas9 editing to recapitulate thyroid cancer histotype progression

Summary: Thyroid carcinoma represents the first malignancy among the endocrine organs. Investigating the cellular hierarchy and the mechanisms underlying the initiation of thyroid carcinoma is crucial in thyroid cancer research. Here, we present a protocol for deriving thyroid cell lineage from huma...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Vincenzo Davide Pantina, Francesco Verona, Alice Turdo, Veronica Veschi, Chiara Modica, Melania Lo Iacono, Miriam Gaggianesi, Sebastiano Di Bella, Matilde Todaro, Simone Di Franco, Giorgio Stassi
Format:	Article
Language:	English
Published:	Elsevier 2024-09-01
Subjects:	Cell culture Cancer CRISPR Cell Differentiation
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724004283