Preservation of functionality, immunophenotype, and recovery of HIV RNA from PBMCs cryopreserved for more than 20 years

BackgroundMany research laboratories have long-term repositories of cryopreserved peripheral blood mononuclear cells (PBMC), which are costly to maintain but are of uncertain utility for immunological studies after decades in storage. This study investigated preservation of cell surface phenotypes a...

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Bibliographic Details

Published in:	Frontiers in Immunology
Main Authors:	Wayne B. Dyer, Kazuo Suzuki, Angelique Levert, Mitchell Starr, Andrew R. Lloyd, John J. Zaunders
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2024-08-01
Subjects:	cryopreservation biobank viability immunophenotyping HIV reactivation T cell subsets
Online Access:	https://www.frontiersin.org/articles/10.3389/fimmu.2024.1382711/full