A comparison of normalization methods for the expression of genes associated with oxidative stress in the liver of sheep

Abstract Background Normalization of data obtained from RT-qPCR studies is important for accurate interpretation of the results. The two most common methods of normalization that are used are the reference gene method or an algorithm-only approach, such as NORMA-Gene. Here, we assessed the impact of...

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Bibliographic Details
Published in:BMC Genomic Data
Main Authors: Sarah Babington, Luoyang Ding, Alan J. Tilbrook, Shane K. Maloney, Dominique Blache
Format: Article
Language:English
Published: BMC 2025-07-01
Subjects:
Ovine
Reference genes
Gene expression
RT-qPCR
NORMA-Gene
Online Access:https://doi.org/10.1186/s12863-025-01345-y

Internet

https://doi.org/10.1186/s12863-025-01345-y

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