Modular Cloning by Golden Gate Assembly and Possible Application in Pathway Design

Preparation of expression vectors using conventional cloning strategies is laborious and not suitable for the design of metabolic pathways or enzyme cascades, which usually requires the preparation of a vector library to identify productive clones. Recently, Modular Cloning as a novel cloning techn...

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Bibliographic Details

Published in:	CHIMIA
Main Authors:	Zrinka Raguz Nakic, Christin Peters
Format:	Article
Language:	German
Published:	Swiss Chemical Society 2023-06-01
Subjects:	Expression control Golden Gate assembly Modular Cloning Pathway design
Online Access:	https://www.chimia.ch/chimia/article/view/6961