Quantitative analysis of RNAscope staining for c-fos expression in mouse brain tissue as a measure of Neuronal Activation

The expression of c-fos mRNA is an indirect marker of neuronal activity. RNAscope ACD Bio RNAscope (now Biotechne) is a proprietary in-situ mRNA detection technology using branched DNA amplification and z paired probes to deliver a robust and specific assay designed primarily for use on formalin fix...

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Bibliographic Details

Published in:	MethodsX
Main Authors:	Cristina Cosi, Michael Millar, Mariana Beltran, Lorcan Sherry, Silvia Gatti-McArthur
Format:	Article
Language:	English
Published:	Elsevier 2021-01-01
Subjects:	Brain c-fos RNAscope in-situ hybridisation
Online Access:	http://www.sciencedirect.com/science/article/pii/S2215016121001412