Construction of L-Asparaginase Stable Mutation for the Application in Food Acrylamide Mitigation

Acrylamide, a II A carcinogen, widely exists in fried and baked foods. L-asparaginase can inhibit acrylamide formation in foods, and enzymatic stability is the key to its application. In this study, the <i>Escherichia coli</i> L-asparaginase (ECA) stable variant, D60W/L211R/L310R, was ob...

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Bibliographic Details

Published in:	Fermentation
Main Authors:	Bing Yuan, Pengfei Ma, Yuxuan Fan, Bo Guan, Youzhen Hu, Yan Zhang, Wenli Yan, Xu Li, Yongqing Ni
Format:	Article
Language:	English
Published:	MDPI AG 2022-05-01
Subjects:	L-asparaginase stability mutation acrylamide food safety
Online Access:	https://www.mdpi.com/2311-5637/8/5/218