BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

Abstract Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands...

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Bibliographic Details

Published in:	Genome Biology
Main Authors:	Daniel Alpern, Vincent Gardeux, Julie Russeil, Bastien Mangeat, Antonio C. A. Meireles-Filho, Romane Breysse, David Hacker, Bart Deplancke
Format:	Article
Language:	English
Published:	BMC 2019-04-01
Subjects:	RNA-seq Transcriptomics Gene expression qPCR Barcoding
Online Access:	http://link.springer.com/article/10.1186/s13059-019-1671-x