A spatial long-read approach at near-single-cell resolution reveals developmental regulation of splicing and polyadenylation sites in distinct cortical layers and cell types

Abstract Genome-wide spatial long-read approaches often lack single-cell resolution and yield limited read lengths. Here, we introduce spatial ISOform sequencing (Spl-ISO-Seq), which reveals exons and polyadenylation sites with near-single-cell resolution. Spl-ISO-Seq selects long cDNAs and doubles...

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Bibliographic Details

Published in:	Nature Communications
Main Authors:	Careen Foord, Andrey D. Prjibelski, Wen Hu, Lieke Michielsen, Andrea Vandelli, Oleksandr Narykov, Brian Evans, Justine Hsu, Natan Belchikov, Julien Jarroux, Yi He, M. Elizabeth Ross, Iman Hajirasouliha, Gian Gaetano Tartaglia, Dmitry Korkin, Alexandru I. Tomescu, Hagen U. Tilgner
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-08-01
Online Access:	https://doi.org/10.1038/s41467-025-63301-9