Development of a rapid and sensitive RPA-CRISPR/Cas12a-based assay for the detection of Brucella melitensis

ABSTRACT Brucellosis, a zoonotic disease caused by Brucella species, presents significant public health challenges due to its complex diagnosis and the limited availability of rapid detection methods. To address these challenges, we developed a novel detection method that integrates recombinase poly...

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Bibliographic Details
Published in:Microbiology Spectrum
Main Authors: Yu Shen, Chenchen Yi, Heng Wang, Yi Tang, Jun Li
Format: Article
Language:English
Published: American Society for Microbiology 2025-10-01
Subjects:
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00998-25