Real-Time PCR Assay for Quantitative Mismatch Detection
We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations. Following PCR or RT-PCR of a gene segment that may contain allele-specific differences, 100 pg amp...
| Published in: | BioTechniques |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-03-01
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| Online Access: | https://www.future-science.com/doi/10.2144/03343st01 |