Locking of 3′ ends of single-stranded DNA templates for improved Pyrosequencing™ performance
In Pyrosequencing™, a DNA strand complementary to a single-stranded DNA (ssDNA) template is synthesized, whereby each incorporated nucleotide yields detectable light, and the light intensity is proportional to the incorporated nucleotides. Correct data interpretation (i.e., signal-to-noise ratio of...
| Published in: | BioTechniques |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2004-07-01
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| Online Access: | https://www.future-science.com/doi/10.2144/04371ST02 |