Protocol for trapping transient endogenous formaldehyde in live cells to visualize its signaling

Summary: Formaldehyde is emerging as a key signaling modulator. Here, we present Trami, a protocol that traps endogenous formaldehyde in live cells and converts its transient signals into stable proximal protein labels for subsequent signaling study. We describe probe design, live-cell administratio...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Yuan Pan, Xing-Guang Liang, Xin Li
Format:	Article
Language:	English
Published:	Elsevier 2025-12-01
Subjects:	Cell-based Assays Microscopy Molecular/Chemical Probes Biotechnology and bioengineering Chemistry
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166725005143