pET expression vector customized for efficient seamless cloning
Here we present a modification of the widely used pET29 expression vector for use in rapid and straightforward parallel cloning via a gene replacement and Golden Gate strategy. The modification can be applied to other expression vectors for Gram-negative bacteria. We have used the modified vectors t...
| Published in: | BioTechniques |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-11-01
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| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2020-0101 |