Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in

Cell lines expressing foreign genes have been widely used to produce a variety of recombinant proteins. However, generating recombinant protein–expressing cell lines is usually a lengthy process and the resulting protein expression levels are often inconsistent. Here, we describe an efficient method...

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發表在:	BioTechniques
Main Authors:	Chiu-An Lo, Alexander W. Greben, Brian Edwin Chen
格式:	Article
語言:	英语
出版:	Taylor & Francis Group 2017-04-01
主題:	protein quantitation reporter (PQR) CRISPR/Cas9 protein production recombinant protein stable cell line
在線閱讀:	https://www.future-science.com/doi/10.2144/000114534