Analysis of Microliter Volumes of Dye-Labeled Nucleic Acids

We describe methodology useful for determining the fluorescent activity of small volumes (e.g., 1 μL) of dye-labeled cDNAs using microabsorbance spectroscopy. A direct relationship was found between the average fluorescent hybridization signal from an array and the fluorescent activity of the cDNA u...

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Bibliographic Details
Published in:BioTechniques
Main Authors: Tateo Nagai, William H. Chapman
Format: Article
Language:English
Published: Taylor & Francis Group 2002-02-01
Online Access:https://www.future-science.com/doi/10.2144/02322pf01