Establishment of a TRPV2 knockout human embryonic stem cell line (WAe009-A-1Y) using episomal vector-based CRISPR/Cas9

We established a TRPV2-knockout human embryonic stem cell line (WAe009-A-1Y) using a non-integrating episomal CRISPR/Cas9 system. This cell line exhibits an 8-nucleotide frameshift deletion in TRPV2 exon 2, confirmed pluripotency (97.6 % SSEA4+ cells, trilineage differentiation), and a normal female...

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Bibliographic Details

Published in:	Stem Cell Research
Main Authors:	Rui Bai, Siyao Zhang, Xiaoyan Gu, Yi You, Xujie Liu
Format:	Article
Language:	English
Published:	Elsevier 2025-09-01
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506125000947