Investigation of MSC potency metrics via integration of imaging modalities with lipidomic characterization
Summary: Mesenchymal stem/stromal cell (MSC) therapies have had limited success so far in clinical trials due in part to heterogeneity in immune-responsive phenotypes. Therefore, techniques to characterize these properties of MSCs are needed during biomanufacturing. Imaging cell shape, or morphology...
| Published in: | Cell Reports |
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| Main Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
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Elsevier
2024-08-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724009082 |
| _version_ | 1850370482290819072 |
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| author | Priyanka Priyadarshani Alexandria Van Grouw Adrian Ross Liversage Kejie Rui Arina Nikitina Kayvan Forouhesh Tehrani Bhavay Aggarwal Steven L. Stice Saurabh Sinha Melissa L. Kemp Facundo M. Fernández Luke J. Mortensen |
| author_facet | Priyanka Priyadarshani Alexandria Van Grouw Adrian Ross Liversage Kejie Rui Arina Nikitina Kayvan Forouhesh Tehrani Bhavay Aggarwal Steven L. Stice Saurabh Sinha Melissa L. Kemp Facundo M. Fernández Luke J. Mortensen |
| author_sort | Priyanka Priyadarshani |
| collection | DOAJ |
| container_title | Cell Reports |
| description | Summary: Mesenchymal stem/stromal cell (MSC) therapies have had limited success so far in clinical trials due in part to heterogeneity in immune-responsive phenotypes. Therefore, techniques to characterize these properties of MSCs are needed during biomanufacturing. Imaging cell shape, or morphology, has been found to be associated with MSC immune responsivity—but a direct relationship between single-cell morphology and function has not been established. We used label-free differential phase contrast imaging and matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to evaluate single-cell morphology and explore relationships with lipid metabolic immune response. In interferon gamma (IFN-γ)-stimulated MSCs, we found higher lipid abundances from the ceramide-1-phosphate (C1P), phosphatidylcholine (PC), LysoPC, and triglyceride (TAG) families that are involved in cell immune function. Furthermore, we identified differences in lipid signatures in morphologically defined MSC subpopulations. The use of single-cell optical imaging coupled with single-cell spatial lipidomics could assist in optimizing the MSC production process and improve mechanistic understanding of manufacturing process effects on MSC immune activity and heterogeneity. |
| format | Article |
| id | doaj-art-e49e2cf5a48945ed8e369bccbdaa582a |
| institution | Directory of Open Access Journals |
| issn | 2211-1247 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Elsevier |
| record_format | Article |
| spelling | doaj-art-e49e2cf5a48945ed8e369bccbdaa582a2025-08-19T23:01:36ZengElsevierCell Reports2211-12472024-08-0143811457910.1016/j.celrep.2024.114579Investigation of MSC potency metrics via integration of imaging modalities with lipidomic characterizationPriyanka Priyadarshani0Alexandria Van Grouw1Adrian Ross Liversage2Kejie Rui3Arina Nikitina4Kayvan Forouhesh Tehrani5Bhavay Aggarwal6Steven L. Stice7Saurabh Sinha8Melissa L. Kemp9Facundo M. Fernández10Luke J. Mortensen11School of Chemical, Materials, and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USASchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USASchool of Chemical, Materials, and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USASchool of Chemical, Materials, and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USASchool of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USABeckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Champaign, IL 61820, USAThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA 30332, USARegenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USAThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA 30332, USAThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA 30332, USASchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USASchool of Chemical, Materials, and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA; Regenerative Bioscience Center, Rhodes Center for ADS, University of Georgia, Athens, GA 30602, USA; Corresponding authorSummary: Mesenchymal stem/stromal cell (MSC) therapies have had limited success so far in clinical trials due in part to heterogeneity in immune-responsive phenotypes. Therefore, techniques to characterize these properties of MSCs are needed during biomanufacturing. Imaging cell shape, or morphology, has been found to be associated with MSC immune responsivity—but a direct relationship between single-cell morphology and function has not been established. We used label-free differential phase contrast imaging and matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to evaluate single-cell morphology and explore relationships with lipid metabolic immune response. In interferon gamma (IFN-γ)-stimulated MSCs, we found higher lipid abundances from the ceramide-1-phosphate (C1P), phosphatidylcholine (PC), LysoPC, and triglyceride (TAG) families that are involved in cell immune function. Furthermore, we identified differences in lipid signatures in morphologically defined MSC subpopulations. The use of single-cell optical imaging coupled with single-cell spatial lipidomics could assist in optimizing the MSC production process and improve mechanistic understanding of manufacturing process effects on MSC immune activity and heterogeneity.http://www.sciencedirect.com/science/article/pii/S2211124724009082CP: MetabolismCP: Immunology |
| spellingShingle | Priyanka Priyadarshani Alexandria Van Grouw Adrian Ross Liversage Kejie Rui Arina Nikitina Kayvan Forouhesh Tehrani Bhavay Aggarwal Steven L. Stice Saurabh Sinha Melissa L. Kemp Facundo M. Fernández Luke J. Mortensen Investigation of MSC potency metrics via integration of imaging modalities with lipidomic characterization CP: Metabolism CP: Immunology |
| title | Investigation of MSC potency metrics via integration of imaging modalities with lipidomic characterization |
| title_full | Investigation of MSC potency metrics via integration of imaging modalities with lipidomic characterization |
| title_fullStr | Investigation of MSC potency metrics via integration of imaging modalities with lipidomic characterization |
| title_full_unstemmed | Investigation of MSC potency metrics via integration of imaging modalities with lipidomic characterization |
| title_short | Investigation of MSC potency metrics via integration of imaging modalities with lipidomic characterization |
| title_sort | investigation of msc potency metrics via integration of imaging modalities with lipidomic characterization |
| topic | CP: Metabolism CP: Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724009082 |
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