Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1

Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specifici...

وصف كامل

التفاصيل البيبلوغرافية
الحاوية / القاعدة:Memorias do Instituto Oswaldo Cruz
المؤلفون الرئيسيون: AL Cândido, ED Bontempo, M Resende
التنسيق: مقال
اللغة:الإنجليزية
منشور في: Fundação Oswaldo Cruz (FIOCRUZ) 2000-04-01
الموضوعات:
الوصول للمادة أونلاين:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015