Challenges and Solutions for Leave-One-Out Biosensor Design in the Context of a Rugged Fitness Landscape

The leave-one-out (LOO) green fluorescent protein (GFP) approach to biosensor design combines computational protein design with split protein reconstitution. LOO-GFPs reversibly fold and gain fluorescence upon encountering the target peptide, which can be redefined by computational design of the LOO...

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Bibliographic Details

Published in:	Sensors
Main Authors:	Shounak Banerjee, Keith Fraser, Donna E. Crone, Jinal C. Patel, Sarah E. Bondos, Christopher Bystroff
Format:	Article
Language:	English
Published:	MDPI AG 2024-10-01
Subjects:	green fluorescent protein computational design chromophore ultrabithorax fibers
Online Access:	https://www.mdpi.com/1424-8220/24/19/6380