Quantifying mitochondrial redox and bilirubin content in intact primary hepatocytes of obese mice using fluorescent reporters
Summary: Assessing the physiological role of H2O2 requires sensitive techniques to quantify H2O2 and antioxidants in live cells. Here, we present a protocol to assess the mitochondrial redox state and unconjugated bilirubin levels in intact live primary hepatocytes from obese mice. We described step...
| Published in: | STAR Protocols |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
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| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723003751 |