Crystal structures of monomeric BsmI restriction endonuclease reveal coordinated sequential cleavage of two DNA strands

Abstract BsmI, a thermophilic Type IIS restriction endonuclease from Bacillus stearothermophilus, presents a unique structural composition, housing two distinct active sites within a single monomer. Recognition of the non-symmetrical 5’-GAATGC-3’ sequence enables precise cleavage of the top and bott...

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Bibliographic Details

Published in:	Communications Biology
Main Authors:	Rémi Sieskind, Sophia Missoury, Clément Madru, Isciane Commenge, Germain Niogret, Marcel Hollenstein, Yannick Rondelez, Ludovic Sauguet, Ahmed Haouz, Pierre Legrand, Marc Delarue
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-03-01
Online Access:	https://doi.org/10.1038/s42003-025-07612-z