Protocol to generate genetically engineered organoid-initiated mouse models of esophageal cancer

Summary: Here, we present a protocol for generating genetically engineered organoid-initiated mouse models of esophageal cancer. We describe steps for isolating mouse esophageal primary cells, organoids culture, construct and virus preparation, and genome editing. We then detail procedures for trans...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Jian Wang, Jiajia Du, Xiangmeng Luo, Linjie Guo, Xiang Li, Ziling Dong, Zhenghao Lu, Xudong Wan, Lu Wang, Hongyu Liu, He Li, Ping Tan, Yanhong Ji, Yuan Li, Yaxin Li, Yushang Yang, Yong Yuan, Longqi Chen, Bing Hu, Yu Liu, Feifei Na, Chong Chen
Format:	Article
Language:	English
Published:	Elsevier 2025-09-01
Subjects:	Cancer Model Organisms Organoids
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166725004344