Rapid establishment of Oct4: EGFP transgenic zebrafish homozygote through gynogenesis for monitoring the pluripotency during induction of pluripotent stem cells

Oct4 was one of the important markers of cellular pluripotency. In this study, by Tol2 transgenic technology, the recombinant plasmids composed of Oct4 promoter DNA and enhanced green fluorescent protein (EGFP) gene are microinjected into zebrafish 1-cell stage embryos, and obtain the EGFP positive...

Full description

Bibliographic Details

Published in:	Reproduction and Breeding
Main Authors:	Wenting Xu, Wen Fu, Mindi Long, Xiudan Yuan, Kaiyue Zhao, Xiaoli Hu, Jinhui Liu, Wenbin Liu, Liangyue Peng, Yamei Xiao
Format:	Article
Language:	English
Published:	KeAi Communications Co. Ltd. 2022-09-01
Subjects:	Oct4 Transgenic fish Homozygote Gynogenesis
Online Access:	http://www.sciencedirect.com/science/article/pii/S2667071222000278