Construction of high-yield inositol-1-phosphate synthase recombinant Escherichia coli and optimization of fermentation conditions for myo-inositol production

In this study, the recombinant plasmid pETDuet-INO1 was constructed by introducing the expression gene INO1 of inositol-1-phosphate synthase (IPS) (a key enzyme in inositol synthesis pathway) cloned from Saccharomyces cerevisiae SC288 by gene engineering technology into plasmid pETDuet-1. The recomb...

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Bibliographic Details

Published in:	Zhongguo niangzao
Main Author:	YI Minghua, REN Guoping, HU Qiong, QIAN Mengmeng
Format:	Article
Language:	English
Published:	Editorial Department of China Brewing 2024-12-01
Subjects:	escherichia coli|inositol-1-phosphate synthase|genetic engineering|myo-inositol|orthogonal experiment|fermentation condition
Online Access:	https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-12-143.pdf