snPATHO-seq, a versatile FFPE single-nucleus RNA sequencing method to unlock pathology archives

Abstract Formalin-fixed paraffin-embedded (FFPE) samples are valuable but underutilized in single-cell omics research due to their low RNA quality. In this study, leveraging a recent advance in single-cell genomic technology, we introduce snPATHO-seq, a versatile method to derive high-quality single...

Full description

Bibliographic Details

Published in:	Communications Biology
Main Authors:	Taopeng Wang, Michael J. Roach, Kate Harvey, Javier Escudero Morlanes, Beata Kiedik, Ghamdan Al-Eryani, Alissa Greenwald, Nikolaos Kalavros, Felipe Segato Dezem, Yuling Ma, Yered H. Pita-Juarez, Kellie Wise, Cyril Degletagne, Anna Elz, Azi Hadadianpour, Jack Johanneson, Fiona Pakiam, Heeju Ryu, Evan W. Newell, Laurie Tonon, Andrew Kohlway, Tingsheng Drennon, Jawad Abousoud, Ryan Stott, Paul Lund, Jens Durruthy, Andres F. Vallejo, Wenyan Li, Robert Salomon, Dominik Kaczorowski, Joanna Warren, Lisa M. Butler, Sandra O’Toole, Jasmine Plummer, Ioannis S. Vlachos, Joakim Lundeberg, Alexander Swarbrick, Luciano G. Martelotto
Format:	Article
Language:	English
Published:	Nature Portfolio 2024-10-01
Online Access:	https://doi.org/10.1038/s42003-024-07043-2