Effects of the turn-back primer on intermediate product generation in isothermal DNA amplification

In DNA amplification, the initial step of copying a target sequence from the template DNA, or the so-called intermediate product generation step, is very important. In examining the turn-back primer (TP)–dependent isothermal DNA amplification (TIA) method, we determined the actual time point of inte...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	Yuki Tanaka, Yasumasa Kimura, Yasumasa Mitani, Yuki Kawai, Alexander Lezhava, Shohei Noma, Michihira Tagami, Jun Kawai, Yoshihide Hayashizaki, Kengo Usui
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2010-12-01
Subjects:	isothermal DNA amplification strand displacement turn-back primer single-molecule amplification 454 sequencing
Online Access:	https://www.future-science.com/doi/10.2144/000113566