Effects of the turn-back primer on intermediate product generation in isothermal DNA amplification

In DNA amplification, the initial step of copying a target sequence from the template DNA, or the so-called intermediate product generation step, is very important. In examining the turn-back primer (TP)–dependent isothermal DNA amplification (TIA) method, we determined the actual time point of inte...

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Bibliographic Details
Published in:BioTechniques
Main Authors: Yuki Tanaka, Yasumasa Kimura, Yasumasa Mitani, Yuki Kawai, Alexander Lezhava, Shohei Noma, Michihira Tagami, Jun Kawai, Yoshihide Hayashizaki, Kengo Usui
Format: Article
Language:English
Published: Taylor & Francis Group 2010-12-01
Subjects:
isothermal DNA amplification
strand displacement
turn-back primer
single-molecule amplification
454 sequencing
Online Access:https://www.future-science.com/doi/10.2144/000113566
Description
Summary:In DNA amplification, the initial step of copying a target sequence from the template DNA, or the so-called intermediate product generation step, is very important. In examining the turn-back primer (TP)–dependent isothermal DNA amplification (TIA) method, we determined the actual time point of intermediate product generation by extrapolating dsDNA amplification curves. Our results indicate that intermediate product creation is the rate-limiting step in TIA, and good TP design is advantageous for improving the intermediate production process.
ISSN:0736-6205
1940-9818

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