Analytical assessment of metagenomic workflows for pathogen detection with NIST RM 8376 and two sample matrices

ABSTRACT We assessed the analytical performance of metagenomic workflows using NIST Reference Material (RM) 8376 DNA from bacterial pathogens spiked into two simulated clinical samples: cerebrospinal fluid (CSF) and stool. Sequencing and taxonomic classification were used to generate signals for eac...

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Bibliographic Details
Published in:Microbiology Spectrum
Main Authors: Jason G. Kralj, Stephanie L. Servetas, Samuel P. Forry, Monique E. Hunter, Jennifer N. Dootz, Scott A. Jackson
Format: Article
Language:English
Published: American Society for Microbiology 2025-04-01
Subjects:
metagenomics
reference material
spike-in
pathogen detection
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02806-24

Internet

https://journals.asm.org/doi/10.1128/spectrum.02806-24

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