An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae

Gene dosage-based expression upregulation suffers from instability and random gene integration. Here, the authors report HapAmp, a method that uses haploinsufficiency as evolutionary force to drive in vivo gene amplification, and demonstrate its applications in protein and biochemical production in...

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Bibliographic Details

Published in:	Nature Communications
Main Authors:	Bingyin Peng, Lygie Esquirol, Zeyu Lu, Qianyi Shen, Li Chen Cheah, Christopher B. Howard, Colin Scott, Matt Trau, Geoff Dumsday, Claudia E. Vickers
Format:	Article
Language:	English
Published:	Nature Portfolio 2022-05-01
Online Access:	https://doi.org/10.1038/s41467-022-30529-8