Seeing Red, Selecting True: RUBY-Reported Seed Marker Streamlines CRISPR-Clean Rice Breeding

Abstract The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein (Cas)9 genome-editing technology has become a cornerstone for generating knockout mutations in plant functional genomics. To obtain genetically stable CRISPR-edited plants, the removal of exogen...

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Bibliographic Details

Published in:	Rice
Main Authors:	Jin-Lei Liu, Tao Yang, Yu-Wei Fu, Zhitian Zhan, Hong Chen, Han Cheng, Jiankun Zhou, Yi-Ming Wang, Li-Jun Tang, Wen-Qiang Chen, Ming-Wei Wu, Dake Zhao, Chun-Ming Liu, Yubing He, Jinxin Liu
Format:	Article
Language:	English
Published:	SpringerOpen 2025-10-01
Subjects:	CRISPR-Cas9 Genome editing Transgene-free Visible marker-assisted selection RUBY
Online Access:	https://doi.org/10.1186/s12284-025-00841-0