Protocol for quantifying N-Myc and global protein translation in neuroblastoma cells using click chemistry on polyvinylidene fluoride membranes

Summary: MYCN amplification is a hallmark of aggressive neuroblastoma, driving N-Myc overexpression and enhancing protein synthesis, making these processes potential therapeutic targets. Here, we present a protocol for quantifying nascent N-Myc and global protein translation in neuroblastoma cells....

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Pamorn Chittavanich, Duangporn Saengwimol, Ariestya Indah Permata Sari, Atthapol Srimongkol, Rossukon Kaewkhaw
Format:	Article
Language:	English
Published:	Elsevier 2024-12-01
Subjects:	Cancer Protein Biochemistry Proteomics
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724005422