Fluorescently labeled oligonucleotide extension: a rapid and quantitative protocol for primer extension
Identification of nucleotides used for RNA chain initiation or for contacting DNA binding proteins is basic to our understanding of gene regulation. Normally, a radioactive primer is used to copy RNA or DNA. The polymerase extension stops at free ends of mRNA (as in promoter mapping) or at the posit...
| Published in: | BioTechniques |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-07-01
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| Online Access: | https://www.future-science.com/doi/10.2144/03351rr01 |