1‐Hydroxy‐xanthine derivatives inhibit the human Caf1 nuclease and Caf1‐containing nuclease complexes via Mg2+‐dependent binding

In eukaryotic cells, cytoplasmic mRNA is characterised by a 3′ poly(A) tail. The shortening and removal of poly(A) tails (deadenylation) by the Ccr4‐Not nuclease complex leads to reduced translational efficiency and RNA degradation. Using recombinant human Caf1 (CNOT7) enzyme as a screening tool, we...

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Bibliographic Details

Published in:	FEBS Open Bio
Main Authors:	Blessing Airhihen, Lorenzo Pavanello, Gopal P. Jadhav, Peter M. Fischer, Gerlof Sebastiaan Winkler
Format:	Article
Language:	English
Published:	Wiley 2019-04-01
Subjects:	1‐hydroxy‐xanthine Caf1/CNOT7 nuclease Ccr4‐Not deadenylase Ribonuclease thermal stability assay
Online Access:	https://doi.org/10.1002/2211-5463.12605