Protocol to establish a stable MLL-AF9_AML mouse model

• Published in: STAR Protocols
• Main Authors: Jun Lu, Huanhuan Zhao, Lingling Yang, Xi Jiang
• Format: Article
• Language: English
• Published: Elsevier 2022-09-01
• Subjects: Cancer; Cell isolation; Cell separation/fractionation; Cell-based Assays
• Online Access: http://www.sciencedirect.com/science/article/pii/S2666166722004397
• ISSN: 2666-1667

Summary: Acute myeloid leukemia (AML) is one of the most common and fatal forms of hematopoietic malignancies. Here, we describe a mouse MLL-AF9_AML model to investigate AML. We have optimized the protocols for retrovirus infection, bone marrow transplantation (BMT), and leukemia monitoring to create a stable mouse model. In particular, we have used two rounds of BMT to enhance stability and efficiency. This model can be used to conduct drug administration and/or other interventions easily.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.