Safe and potent anti‐CD19 CAR T‐cells with shRNA‐IL‐6 gene silencing element in patients with refractory or relapsed B‐cell acute lymphoblastic leukemia

Abstract Severe cytokine release syndrome (sCRS) and immune effector cell‐associated neurotoxicity syndrome (ICANS) have limited the widespread use of chimeric antigen receptor T (CAR T)‐cell therapy. We designed a novel anti‐CD19 CAR (ssCART‐19) with a small hairpin RNA (shRNA) element to silence t...

Full description

Bibliographic Details
Published in:	HemaSphere
Main Authors:	Jin‐Feng Ma, Jia‐Wei Yan, Mei‐Jing Liu, Chun‐Long Yan, Xiao‐Wen Tang, Hui‐Ying Qiu, Miao Miao, Yue Han, Li‐Min Li, Li‐Qing Kang, Nan Xu, Zhou Yu, Jing‐Wen Tan, Hong‐Jia Zhu, Xu Jia, Zhi‐Zhi Zhang, Miao Wang, Hai‐Ping Dai, Lei Yu, Sheng‐Li Xue, De‐Pei Wu, Wen‐Jie Gong
Format:	Article
Language:	English
Published:	Wiley 2024-10-01
Online Access:	https://doi.org/10.1002/hem3.70007

Similar Items

shRNA-mediated gene silencing of HDAC11 empowers CAR-T cells against prostate cancer
by: Hongmei Zhang, et al.
Published: (2024-05-01)

146 Evolving Mutliplexed shRNA to generate tailored CAR T cell therapy
by: David Gilham, et al.
Published: (2021-11-01)

Silencing Human Rb2/p130 with shRNA
by: Eleonora Leucci, et al.
Published: (2007-01-01)

Synthetic Pre-miRNA-Based shRNA as Potent RNAi Triggers
by: Kazuya Terasawa, et al.
Published: (2011-01-01)

Silencing of the hTERT gene by shRNA inhibits colon cancer SW480 cell growth in vitro and in vivo.
by: Ai-Qun Liu, et al.
Published: (2014-01-01)

Delivery of therapeutic AGT shRNA by PEG-Bu for hypertension therapy.
by: Yu-Qiang Wang, et al.
Published: (2013-01-01)

The influence of survivin shRNA on the cell cycle and the invasion of SW480 cells of colorectal carcinoma
by: Yu Jin, et al.
Published: (2008-07-01)

ShRNA-targeted centromere protein A inhibits hepatocellular carcinoma growth.
by: Yongmei Li, et al.
Published: (2011-01-01)

Effect of shRNA Mediated Silencing of YB-1 Protein on the Expression of Matrix Collagenases in Malignant Melanoma Cell In Vitro
by: Wisam Nabeel Ibrahim, et al.
Published: (2018-01-01)

Effect of Lentivirus-induced shRNA Silencing CXCR4 Gene on Proliferation and Apoptosis in Human Esophageal Carcinoma Cell Line Eca109
by: Dao-feng WANG, et al.
Published: (2010-06-01)

Oct4 and Hypoxia Dual-Regulated Oncolytic Adenovirus Armed with shRNA-Targeting Dendritic Cell Immunoreceptor Exerts Potent Antitumor Activity against Bladder Cancer
by: Che-Yuan Hu, et al.
Published: (2023-09-01)

Lentiviral-Encoded shRNA Silencing of Proteoglycan Decorin Enhances Tendon Repair and Regeneration within a Rat Model
by: Ping Lu, et al.
Published: (2013-09-01)

ShRNA-mediated BMI-1 gene silencing inhibits gastrointestinal stromal tumor cell telomerase activity and enhances apoptosis
by: Xin Wang, et al.
Published: (2018-11-01)

Non-targeting control for MISSION shRNA library silences SNRPD3 leading to cell death or permanent growth arrest
by: Maria Czarnek, et al.
Published: (2021-12-01)

298 A miRNA-based shRNA platform for the easy, safe, and effective generation of allogeneic CAR T-cells
by: Thủy Nguyễn, et al.
Published: (2023-11-01)

Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells
by: Lin Liu, et al.
Published: (2011-01-01)

Multiplex shRNA Screening of Germ Cell Development by in Vivo Transfection of Mouse Testis
by: Nicholas R. Y. Ho, et al.
Published: (2017-01-01)

Analysis benefits of a second Allo-HSCT after CAR-T cell therapy in patients with relapsed/refractory B-cell acute lymphoblastic leukemia who relapsed after transplant
by: Xing-yu Cao, et al.
Published: (2023-07-01)

307 CAR-Macrophages with custom intronic shRNA exhibit enhanced efficacy against solid tumors
by: Nicholas Anderson, et al.
Published: (2023-11-01)

A simple and robust vector-based shRNA expression system used for RNA interference.
by: Xue-jun Wang, et al.
Published: (2013-01-01)

Follicle-stimulating hormone peptide-conjugated nanoparticles for targeted shRNA delivery lead to effective gro-α silencing and antitumor activity against ovarian cancer
by: Shan-Shan Hong, et al.
Published: (2018-01-01)

Control of Aedes albopictus populations by silencing of the vesicular GABA transporter (vgat) and the vesicular monoamine transporter (vmat) genes using recombinant Chlorella shRNA
by: Xiaodong Deng, et al.
Published: (2025-10-01)

Analysis of Processing, Post-Maturation, and By-Products of shRNA in Gene and Cell Therapy Applications
by: Zhenyi Hong, et al.
Published: (2025-04-01)

Efficient and nontoxic biological response carrier delivering TNF-α shRNA for gene silencing in a murine model of rheumatoid arthritis
by: Jialin Song, et al.
Published: (2016-08-01)

285 Proof-of-concept of a non-gene editing technology using shRNA down-regulation to engineer and optimize CAR T-cell functionality
by: Thủy Nguyễn, et al.
Published: (2023-11-01)

shRNA‑mediated knockdown of KNTC1 inhibits non-small-cell lung cancer through regulating PSMB8
by: Ruijun Liu, et al.
Published: (2022-08-01)

CAR T-cell therapy for a relapsed/refractory acute B-cell lymphoblastic lymphoma patient in the context of Li-Fraumeni syndrome
by: Yi Xiao, et al.
Published: (2020-05-01)

Alternative approach to generate shRNA from cDNA
by: Anh Dinh, et al.
Published: (2005-04-01)

Stable expression of shRNA for the control of recombinant adenovirus replication
by: M.V.G. Lana, et al.
Published: (2023-07-01)

Optimization of duplex stability and terminal asymmetry for shRNA design.
by: Olga V Matveeva, et al.
Published: (2010-01-01)

Improved siRNA/shRNA functionality by mismatched duplex.
by: Haoquan Wu, et al.
Published: (2011-01-01)

Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response
by: N.N. Dang, et al.
Published: (2015-01-01)

A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing
by: Benoit Moindrot, et al.
Published: (2015-07-01)

STAT3-specific nanocarrier for shRNA/drug dual delivery and tumor synergistic therapy
by: Le Sun, et al.
Published: (2024-11-01)

Targeted shRNA screening identified critical roles of pleckstrin-2 in erythropoiesis
by: Baobing Zhao, et al.
Published: (2014-07-01)

Three new shRNA expression vectors targeting the CYP3A4 coding sequence to inhibit its expression
by: Siyun Xu, et al.
Published: (2014-10-01)

Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus
by: Fanfan Li, et al.
Published: (2019-06-01)

Artemether combined with shRNA interference of vascular cell adhesion molecule-1 significantly inhibited the malignant biological behavior of human glioma cells.
by: Ying-Bin Wang, et al.
Published: (2013-01-01)

Expression of plasmid-based shRNA against the E1 and nsP1 genes effectively silenced Chikungunya virus replication.
by: Shirley Lam, et al.
Published: (2012-01-01)

Preparation of ShRNA-LDH nanoparticle and its application in biological control of Mikania micrantha
by: MO Yilin, et al.
Published: (2023-03-01)