Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation

Kinases are essential cell signaling enzymes that phosphorylate protein substrates using ATP as the universal cosubstrate. A wide variety of ATP analogs have been used in kinase research, although the studies are limited by the cell impermeability of ATP. Here we describe the use of the cationic pol...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	Ahmed E Fouda, D Maheeka Embogama, Vindya Ramanayake-Mudiyanselage, Mary Kay H Pflum
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2018-09-01
Online Access:	https://www.future-science.com/doi/10.2144/btn-2018-0076