Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation
Kinases are essential cell signaling enzymes that phosphorylate protein substrates using ATP as the universal cosubstrate. A wide variety of ATP analogs have been used in kinase research, although the studies are limited by the cell impermeability of ATP. Here we describe the use of the cationic pol...
| Published in: | BioTechniques |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-09-01
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2018-0076 |
| _version_ | 1849811323305590784 |
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| author | Ahmed E Fouda D Maheeka Embogama Vindya Ramanayake-Mudiyanselage Mary Kay H Pflum |
| author_facet | Ahmed E Fouda D Maheeka Embogama Vindya Ramanayake-Mudiyanselage Mary Kay H Pflum |
| author_sort | Ahmed E Fouda |
| collection | DOAJ |
| container_title | BioTechniques |
| description | Kinases are essential cell signaling enzymes that phosphorylate protein substrates using ATP as the universal cosubstrate. A wide variety of ATP analogs have been used in kinase research, although the studies are limited by the cell impermeability of ATP. Here we describe the use of the cationic polymer deacetylated chitosan to permeabilize ATP analogs for live cell applications, including kinase-catalyzed biotinylation. |
| format | Article |
| id | doaj-art-e8bebbda522e49eea226fe2da1d159d8 |
| institution | Directory of Open Access Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| spelling | doaj-art-e8bebbda522e49eea226fe2da1d159d82025-08-20T01:34:48ZengTaylor & Francis GroupBioTechniques0736-62051940-98182018-09-0165314314810.2144/btn-2018-0076Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylationAhmed E Fouda0D Maheeka Embogama1Vindya Ramanayake-Mudiyanselage2Mary Kay H Pflum31Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA1Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA1Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA1Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USAKinases are essential cell signaling enzymes that phosphorylate protein substrates using ATP as the universal cosubstrate. A wide variety of ATP analogs have been used in kinase research, although the studies are limited by the cell impermeability of ATP. Here we describe the use of the cationic polymer deacetylated chitosan to permeabilize ATP analogs for live cell applications, including kinase-catalyzed biotinylation.https://www.future-science.com/doi/10.2144/btn-2018-0076 |
| spellingShingle | Ahmed E Fouda D Maheeka Embogama Vindya Ramanayake-Mudiyanselage Mary Kay H Pflum Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation |
| title | Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation |
| title_full | Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation |
| title_fullStr | Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation |
| title_full_unstemmed | Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation |
| title_short | Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation |
| title_sort | chitosan assisted permeabilization of atp biotin for live cell kinase catalyzed biotinylation |
| url | https://www.future-science.com/doi/10.2144/btn-2018-0076 |
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