Genetic and biochemical characterization of a radical SAM enzyme required for post-translational glutamine methylation of methyl-coenzyme M reductase

ABSTRACT Methyl-coenzyme M reductase (MCR), the key catalyst in the anoxic production and consumption of methane, contains an unusual 2-methylglutamine residue within its active site. In vitro data show that a B12-dependent radical SAM (rSAM) enzyme, designated MgmA, is responsible for this post-tra...

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Bibliographic Details

Published in:	mBio
Main Authors:	Roy J. Rodriguez Carrero, Cody T. Lloyd, Janhavi Borkar, Shounak Nath, Liviu M. Mirica, Satish Nair, Squire J. Booker, William Metcalf
Format:	Article
Language:	English
Published:	American Society for Microbiology 2025-02-01
Subjects:	methyl-coenzyme M reductase methanogenesis post-translational modification archaea Methanosarcina
Online Access:	https://journals.asm.org/doi/10.1128/mbio.03546-24