exoCasMINI: A T5 exonuclease fused CRISPR-Cas12f system with enhanced gene editing efficiency

Summary: CRISPR-Cas9 and Cas12a are widely used for genome editing, but their large size limits delivery efficiency. Compact Cas12f proteins offer delivery advantages but suffer from low activity. To address this limitation, we engineered an enhanced Cas12f system (exoCasMINI) by fusing T5 exonuclea...

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Bibliographic Details

Published in:	iScience
Main Authors:	Kaitong Liao, Kechen Chen, Shufeng Ma, Xiaofeng Yang, Peihan Chen, Sijie Li, Mengrao Li, Xin Zhang, Ying Lin
Format:	Article
Language:	English
Published:	Elsevier 2025-08-01
Subjects:	Enzymology Biochemistry methods Techniques in genetics Biochemistry applications
Online Access:	http://www.sciencedirect.com/science/article/pii/S2589004225014324