Systematic Exploration of Optimized Base Editing gRNA Design and Pleiotropic Effects with BExplorer

Base editing technology is being increasingly applied in genome engineering, but the current strategy for designing guide RNAs (gRNAs) relies substantially on empirical experience rather than a dependable and efficient in silico design. Furthermore, the pleiotropic effect of base editing on disease...

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Bibliographic Details

Published in:	Genomics, Proteomics & Bioinformatics
Main Authors:	Gongchen Zhang, Chenyu Zhu, Xiaohan Chen, Jifang Yan, Dongyu Xue, Zixuan Wei, Guohui Chuai, Qi Liu
Format:	Article
Language:	English
Published:	Oxford University Press 2023-12-01
Subjects:	Gene editing Base editing Pleiotropy CRISPR/Cas9 gRNA design
Online Access:	http://www.sciencedirect.com/science/article/pii/S1672022922000821