Cy3-ATP labeling of unfixed, permeabilized mouse hair cells
Abstract ATP-utilizing enzymes play key roles in hair bundles, the mechanically sensitive organelles of sensory hair cells in the inner ear. We used a fluorescent ATP analog, EDA-ATP-Cy3 (Cy3-ATP), to label ATP-binding proteins in two different preparations of unfixed hair-cell stereocilia of the mo...
| Published in: | Scientific Reports |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-12-01
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| Online Access: | https://doi.org/10.1038/s41598-021-03365-x |