Cy3-ATP labeling of unfixed, permeabilized mouse hair cells

Abstract ATP-utilizing enzymes play key roles in hair bundles, the mechanically sensitive organelles of sensory hair cells in the inner ear. We used a fluorescent ATP analog, EDA-ATP-Cy3 (Cy3-ATP), to label ATP-binding proteins in two different preparations of unfixed hair-cell stereocilia of the mo...

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Bibliographic Details
Published in:	Scientific Reports
Main Authors:	Itallia V. Pacentine, Peter G. Barr-Gillespie
Format:	Article
Language:	English
Published:	Nature Portfolio 2021-12-01
Online Access:	https://doi.org/10.1038/s41598-021-03365-x

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