Determination of Tropomyosin in Shrimp and Crab by Liquid Chromatography–Tandem Mass Spectrometry Based on Immunoaffinity Purification

A UPLC-MS/MS method was developed for the detection of tropomyosin (TM) in shrimp and crab. After simple extraction, the samples were purified by immunoaffinity column and then digested by trypsin. The obtained sample was separated by Easy-nLC 1000-Q Exactive. The obtained spectrums were analyzed by...

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الحاوية / القاعدة:	Frontiers in Nutrition
المؤلفون الرئيسيون:	Sufang Fan, Junmei Ma, Chunsheng Li, Yanbo Wang, Wen Zeng, Qiang Li, Jinru Zhou, Liming Wang, Yi Wang, Yan Zhang
التنسيق:	مقال
اللغة:	الإنجليزية
منشور في:	Frontiers Media S.A. 2022-03-01
الموضوعات:	liquid chromatography-tandem mass spectrometry tropomyosin immunoaffinity purification isotope-label signature peptide
الوصول للمادة أونلاين:	https://www.frontiersin.org/articles/10.3389/fnut.2022.848294/full