Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces

Cattle are considered to be the main reservoir for Shiga toxin-producing Escherichia coli (STEC) and are often the direct or indirect source of STEC outbreaks in humans. Accurate measurement of the concentration of shed STEC in cattle feces could be a key answer to questions concerning transmission...

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Bibliographic Details

Published in:	Toxins
Main Authors:	Bavo Verhaegen, Koen De Reu, Lieven De Zutter, Karen Verstraete, Marc Heyndrickx, Els Van Coillie
Format:	Article
Language:	English
Published:	MDPI AG 2016-05-01
Subjects:	Shiga Toxin-producing Escherichia coli real-time qualitative PCR droplet digital PCR PCR inhibition cattle
Online Access:	http://www.mdpi.com/2072-6651/8/5/157