Protocol for cryopreserving neural organoids and human living brain tissue using MEDY

Summary: Cryopreservation of neural organoids and human living brain tissue faces huge challenges. Here, we present a cryopreservation protocol for the reliable storage of diverse neural organoids and human living brain tissue using methylcellulose, ethylene glycol, DMSO, and Y27632, termed MEDY. We...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Weiwei Xue, Jiacheng Du, Yunteng Li, Zhicheng Shao
Format:	Article
Language:	English
Published:	Elsevier 2024-12-01
Subjects:	neuroscience organoids stem cells
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724006312